Paste a protein sequence and pick a host to get a codon-optimized transcription unit with a selection marker — as a linear cassette or a complete circular plasmid.
From a protein sequence and a host to a finished cassette, five stages annotate each step in this entry.
Paste an amino-acid sequence and choose one of 14 host organisms.
The CDS is codon-optimized for the host and scored for CAI, GC, and rare codons.
Promoter, RBS, and terminator are picked per host, each with a cited source.
A transcription unit is assembled with a selection marker — linear or circular.
Download FASTA/GenBank, copy the sequence, and save the run to your workspace.
Bacteria, yeast, filamentous fungi, mammalian, insect, plant, and cell-free systems.
CAI before→after, GC content, rare codons, and constraint checks for the chosen host.
Every promoter, RBS, and terminator carries a literature source with a verified flag.
A cassette with a selection marker, or a full plasmid built on a host backbone.
Enter a protein sequence, choose a host, then design the cassette.
Watch the design advance stage by stage.
The assembled cassette with part selection, codon report, feature map, primers, and traceable sources.
Each saved run becomes a reproducible entry with an Entry ID you can cite in your methods. Hand off the chosen parts to Multi-gene Assembly to build a larger construct.
Run a design first, then save it here.